J'en veux pour preuve que le chiffre de 677 avancé pour M. Pneumoniae n'est plus exact. Une mise à jour a été éffectuée, mise à jour que je me suis - temporairement et volontairement - gardé d'annoncer puisque je voulais me donner l'occasion de vérifier sa vigilance en matière de référence scientifique. Elle est NULLE !
La nouille glosait sur M. Pneumoniae mais je ne me souviens pas (ai-je tort ?) l'avoir entendu parler de M.Genitalium avant que j'en parle (encore une fois ai-je tort ?) qui n'en compte que 479 [oui-oui Juju ! On sait (et depuis longtemps): C'est un parasite]. Il a fallu qu'on lui en apporte la preuve.
Même chose pour le chiffre de 250, qui l'a d'abord fait bondir mais qu'il utilise maintenant dans les discussions.
Bref la nouille est à la remorque des membres de ce forum pour les références et les "investigations".
Pourtant avec son "manuel universitaire", il ne devrait pas y avoir de problème non ?
Le chiffre que Juju utilisera désormais pour M. Pneumoniae est de 688
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Nucleic Acids Res 2000 Sep 1;28(17):3278-88
Re-annotating the Mycoplasma pneumoniae genome sequence: adding value, function and reading frames.
Dandekar T, Huynen M, Regula JT, Ueberle B, Zimmermann CU, Andrade MA, Doerks T, Sanchez-Pulido L, Snel B, Suyama M, Yuan YP, Herrmann R, Bork P.
EMBL, Postfach 102209, D-69012 Heidelberg, Germany, Max Delbruck Centre for Molecular Medicine, Robert-Rossle-Strabetae 10, 13092 Berlin-Buch, Germany.
Four years after the original sequence submission, we have re-annotated the genome of Mycoplasma pneumoniae to incorporate novel data. The total number of ORFss has been increased from 677 to 688 (10 new proteins were predicted in intergenic regions, two further were newly identified by mass spectrometry and one protein ORF was dismissed) and the number of RNAs from 39 to 42 genes. For 19 of the now 35 tRNAs and for six other functional RNAs the exact genome positions were re-annotated and two new tRNA(Leu) and a small 200 nt RNA were identified. Sixteen protein reading frames were extended and eight shortened. For each ORF a consistent annotation vocabulary has been introduced. Annotation reasoning, annotation categories and comparisons to other published data on M.pneumoniae functional assignments are given. Experimental evidence includes 2-dimensional gel electrophoresis in combination with mass spectrometry as well as gene expression data from this study. Compared to the original annotation, we increased the number of proteins with predicted functional features from 349 to 458. The increase includes 36 new predictions and 73 protein assignments confirmed by the published literature. Furthermore, there are 23 reductions and 30 additions with respect to the previous annotation. mRNA expression data support transcription of 184 of the functionally unassigned reading frames.
Texte complet à:
http://nar.oupjournals.org/cgi/content/full/28/17/3278
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